Principle
Separate before evaluating.
Chromatography distributes sample components between a stationary and a mobile phase. In modern liquid chromatography, the sample moves through a chromatographic system and separated components are detected.
Analytical Methods
HPLC
High-performance liquid chromatography separates components of a sample chromatographically. In peptide analytics, HPLC is commonly used for method-linked assessment of purity and secondary components.
In a peptide report
The context belongs with the value.
AXIOM presents a purity value only together with batch, laboratory, test date and the actual report. The number is not copied from supplier marketing or another batch.
Read the report
Five points to check first.
Sample / batch
The report must clearly correspond to the tested sample or batch.
Method
Column, mobile phase, detection and other method parameters determine how separation is achieved.
Chromatogram
Peaks represent detected components at specific retention times.
Main peak & secondary peaks
An area-percent value is method-dependent and does not automatically establish identity, amount or sterility.
Acceptance criterion
A limit is meaningful only when it belongs to the validated method and the specific product specification.